cell cultures human colon cancer cell line sw480  (ATCC)

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) LRP5 protein expression in SW480 (A) and LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus and treated with 10 μg/mL cycloheximide (CHX) for 0, 2, and 4 h. The whole-cell lysates were analyzed using western blot.

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Stable Transfection, Transduction, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A) SNX17, LRP5, and active β-catenin expression in 293T or SW480 cells transfected with control vector (Ctrl) or FLAG-tagged SNX17 expression vector for 48 h, then processed for western blot analysis.

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) SW480 (A) or LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus were treated with the PI3P biosynthesis inhibitor VPS34-IN-1 (1 μM) for 2 h and processed for the indicated protein expression using western blot analysis.

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Stable Transfection, Transduction, Expressing, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Generated, Plasmid Preparation, Recombinant, Modification, Western Blot, Protease Inhibitor, Cell Viability Assay, Magnetic Beads, Expressing, Software

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) LRP5 protein expression in SW480 (A) and LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus and treated with 10 μg/mL cycloheximide (CHX) for 0, 2, and 4 h. The whole-cell lysates were analyzed using western blot.

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Stable Transfection, Transduction, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A) SNX17, LRP5, and active β-catenin expression in 293T or SW480 cells transfected with control vector (Ctrl) or FLAG-tagged SNX17 expression vector for 48 h, then processed for western blot analysis.

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) SW480 (A) or LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus were treated with the PI3P biosynthesis inhibitor VPS34-IN-1 (1 μM) for 2 h and processed for the indicated protein expression using western blot analysis.

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Stable Transfection, Transduction, Expressing, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Generated, Plasmid Preparation, Recombinant, Modification, Western Blot, Protease Inhibitor, Cell Viability Assay, Magnetic Beads, Expressing, Software

Journal: Clinical and experimental medicine

Article Title: Knockdown of MMP-7 inhibits cell proliferation and enhances sensitivity to 5-Fluorouracil and X-ray irradiation in colon cancer cells.

doi: 10.1007/s10238-012-0212-7

Figure Lengend Snippet: Fig. 2 Down regulation of MMP-7 expression inhibited the cancer cells’ growth and colony formation. a By MTT assay, LV-MMP- 7shRNA3 cells disclosed a significant decreaseinproliferation compared with LV-con and WT SW480 cell cells (*P \ 0.05, ANOVA and LSD test). b Colony formation assay further revealed LV-MMP-7shRNA3 cells had lower positive colony formation than LV-con and WT cells (*P\ 0.05, ANOVA and LSD test). There was no significant difference between LV-con and untreated cell groups (*P [ 0.05, ANOVA and LSD test). Data shown represent at least three independent experiments

Article Snippet: Cell culture Human colon cancer cell line SW480 and 293 T cells were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA) and maintained in DMEM medium (Genom BioMed Technology, Inc. Hangzhou, China) supplemented with 2 % penicillin–streptomycin, 1.5 mM L-glutamine, 2 % NaHCO3 and 10 % fetal bovine serum in a humidified atmosphere of 5 % CO2 at 37 C. Lentivirus-mediated RNA interference We designed and cloned a shRNA template into a thirdgeneration self-inactivating lentivirus vector containing a CMV-driven GFP reporter and a U6 promoter upstream of the cloning restriction sites (HpaI and XhoI).

Techniques: Expressing, MTT Assay, Colony Assay

Journal: Clinical and experimental medicine

Article Title: Knockdown of MMP-7 inhibits cell proliferation and enhances sensitivity to 5-Fluorouracil and X-ray irradiation in colon cancer cells.

doi: 10.1007/s10238-012-0212-7

Figure Lengend Snippet: Fig. 4 Effect of reduced MMP-7 expression in G1/S cell cycle checkpoint control in SW480 cells by flow cytometry analysis. G1/S rate in SW480 cells with reduced MMP-7 expression was signifi- cantly higher than that of LV-Con or WT *(P \ 0.05, Chi-square test). Data shown represent at least three independent experiments

Article Snippet: Cell culture Human colon cancer cell line SW480 and 293 T cells were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA) and maintained in DMEM medium (Genom BioMed Technology, Inc. Hangzhou, China) supplemented with 2 % penicillin–streptomycin, 1.5 mM L-glutamine, 2 % NaHCO3 and 10 % fetal bovine serum in a humidified atmosphere of 5 % CO2 at 37 C. Lentivirus-mediated RNA interference We designed and cloned a shRNA template into a thirdgeneration self-inactivating lentivirus vector containing a CMV-driven GFP reporter and a U6 promoter upstream of the cloning restriction sites (HpaI and XhoI).

Techniques: Expressing, Control, Cytometry